Deep-Learning-AI-on-HPC-clusters

1 Image patch extraction
2 Prediction
3 Heatmap stitching
4 Retreiving run-time statistics

1 Image patch extraction

• Adjust configuration parameters in files, config_testing_cn_true.txt, config_normal_cn_true.txt, config_tumor_cn_true.txt, config_testing.txt, config_normal.txt and config_tumor.txt.

• Run the following commands to launch Son of Grid Engine (SGE) jobs to extract, group patches in HDF5 files and create a lookup table for every HDF5 file.

  1.1 With color normalization

• qsub split_main.sh ./config_testing_cn_true.txt

• qsub split_main.sh ./config_normal_cn_true.txt

• qsub split_main.sh ./config_tumor_cn_true.txt

1.2 Without color normalization

• qsub split_main.sh ./config_testing.txt
  
• qsub split_main.sh ./config_normal.txt
  
• qsub split_main.sh ./config_tumor.txt
  

1.3 Creating lookup tables

• bash create_lookup_grp.sh ./config_testing.txt
  
• bash create_lookup_grp.sh ./config_normal.txt
  
• bash create_lookup_grp.sh ./config_tumor.txt
  

The lookup tables are created only once.

2 Prediction

Run the below commands to launch SGE jobs to generate prediction matrices.

2.1 With color normalization

• qsub process_main.sh ./config_testing_cn_true.txt
  
• qsub process_main.sh ./config_normal_cn_true.txt
  
• qsub process_main.sh ./config_tumor_cn_true.txt
  

2.2 Without color normalization

• qsub process_main.sh ./config_testing.txt
  
• qsub process_main.sh ./config_normal.txt
  
• qsub process_main.sh ./config_tumor.txt
  

3 Heatmap stitching

After the predictions matrices have been generated an SGE job using heatmap_main.sh SGE scrip could be launched to genertae heatmaps. Two arguments for this launch are: a) type of the slides (test, normal or tumor); b) the root directory of the results, like in below example run:

• qsub heatmap_main.sh test /scratch/mikem/UserSupport/weizhe.li/runs_process_cn_True/testing_wnorm_448_400_7690953
  

4 Retreiving run-time statistics

In time_all_stats_pred.sh file adjust job results root directory, DIR and slides type, PREFIX (normal, test or tumor), like below:

• DIR=/scratch/mikem/UserSupport/weizhe.li/runs_process_cn_False/normal_wnorm_448_400_7691563
  
• PREFIX=normal
  Then run:
  
• time bash ./time_all_stats_pred.sh