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readme.md

1 Image patch extraction
2 Prediction
3 Heatmap stitching
4 Retreiving run-time statistics

1 Image patch extraction

The following commands launch Son of Grid Engine (SGE) jobs to extract, group patches in HDF5 files and create a lookup table for every HDF5 file.

1.1 With color normalization

  • qsub split_main.sh ./config_testing_cn_true.txt
  • qsub split_main.sh ./config_normal_cn_true.txt
  • qsub split_main.sh ./config_tumor_cn_true.txt

1.2 Without color normalization

  • qsub split_main.sh ./config_testing.txt
  • qsub split_main.sh ./config_normal.txt
  • qsub split_main.sh ./config_tumor.txt

1.3 Creating lookup tables

  • bash create_lookup_grp.sh ./config_testing.txt
  • bash create_lookup_grp.sh ./config_normal.txt
  • bash create_lookup_grp.sh ./config_tumor.txt

The lookup tables are created only once.

2 Prediction

The following commands launch SGE jobs to generate prediction matrices.

2.1 With color normalization

  • qsub process_main.sh ./config_testing_cn_true.txt
  • qsub process_main.sh ./config_normal_cn_true.txt
  • qsub process_main.sh ./config_tumor_cn_true.txt

2.2 Without color normalization

  • qsub process_main.sh ./config_testing.txt
  • qsub process_main.sh ./config_normal.txt
  • qsub process_main.sh ./config_tumor.txt

3 Heatmap stitching

After the predictions matrices have been generated an SGE job using heatmap_main.sh SGE scrip could be launched to genertae heatmaps. Two arguments for this launch are: a) type of the slides (test, normal or tumor); b) the root directory of the results, like in below example run:

  • qsub heatmap_main.sh test /scratch/mikem/UserSupport/weizhe.li/runs_process_cn_True/testing_wnorm_448_400_7690953

4 Retreiving run-time statistics

In time_all_stats_pred.sh file adjust job results root directory, DIR and slides type, PREFIX (normal, test or tumor), like below:

  • DIR=/scratch/mikem/UserSupport/weizhe.li/runs_process_cn_False/normal_wnorm_448_400_7691563
  • PREFIX=normal Then run:
  • time bash ./time_all_stats_pred.sh